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anti wnt2 santa cruz  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti wnt2 santa cruz
    Anti Wnt2 Santa Cruz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt+2/Wnt-2+Antibody/pm41606293-651-233-234
    Average 91 stars, based on 34 article reviews
    anti wnt2 santa cruz - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Western Blot:

    Article Title: High Fat-High Fructose Diet Elicits Hypogonadotropism Culminating in Autophagy-Mediated Defective Differentiation of Ovarian Follicles
    Article Snippet: Polyvinylidene difluoride (PVDF) membrane was purchased from Millipore, Bangalore, IN.Polyvinylidene difluoride (PVDF) membrane was purchased from Millipore, Bangalore, IN.. Primary antibodies ERα (SC-542), ERβ (SC-8974), AR (SC-815), GnRHR (SC-8682), PR (SC-398898), PRLR (SC-20992), wnt-2 (SC-514382), β-catenin (SC-797), Inhibin (SC-6306), CYP17A1(SC-46080), CYP11A1 (SC-18040), CYP19 (SC-14245), FSHR (SC-13935), LHR (SC-25828), StAR (SC-23523), 3βHSD (SC-30820), and 17βHSD (SC-26968) were purchased from Santacruz Biotechnology (TX, USA).. ATG5 (CST 12994S), ATG12 (CST 4180S), Beclin-1 (CST 3495S), LC3-II (CST 12741S), SQSMT/p62 (95697S), GSK-3β (CST 9315), and pGSK-3β (CST 9336), were purchased from Cell Signaling Technologies, MA, USA.ATG5 (CST 12994S), ATG12 (CST 4180S), Beclin-1 (CST 3495S), LC3-II (CST 12741S), SQSMT/p62 (95697S), GSK-3β (CST 9315), and pGSK-3β (CST 9336), were purchased from Cell Signaling Technologies, MA, USA.

    Article Title: The expressions of Wnt/β-catenin pathway-related components in brainstem gliomas.
    Article Snippet: Sections were then incubated with primary antibodies overnight at 4℃ in a humidified chamber.Sections were then incubated with primary antibodies overnight at 4℃ in a humidified chamber.. The primary antibodies against Wnt-1 (rabbit polyclonal antibody, Abcam, Cambridgeshire, UK), Wnt-2 (goat polyclonal antibody, R&D, Minnesota, USA) and C-myc (mouse monoclonal antibody, Santa Cruz, California, USA) were diluted to 1:50, 1:20 and 1:50, respectively.. The anti-β-catenin and antiKi-67 antibody (mouse monoclonal antibody, Millipore, Massachusetts, USA) were used at a 1:60 and 1:100 dilution.The anti-β-catenin and antiKi-67 antibody (mouse monoclonal antibody, Millipore, Massachusetts, USA) were used at a 1:60 and 1:100 dilution.

    Article Title: Dynamic O-GlcNAc modification regulates CREB-mediated gene expression and memory formation
    Article Snippet: Neurite outgrowth.Neurite outgrowth.. Neurons were electroporated with wild-type or S40A pLEMPRA, pMaxGFP, pcDNA3-Dkk-1-Flag or Ncad(intra) vectors and Wnt-2 (Santa Cruz), CRTC1 or scramble (Invitrogen) siRNA as indicated in the individual figures and then plated at a density of 25,000 neurons cm−2.. Neurons from B6.129-Ogttm1Gwh/J mice (Jackson Laboratories) were electroporated with pMaxGFP, the CRE recombinase pBOB-CAG-iCRE-s.d. (Addgene) or pA2UCOE-OGT vector, along with scramble or CREB siRNA as indicated.Neurons from B6.129-Ogttm1Gwh/J mice (Jackson Laboratories) were electroporated with pMaxGFP, the CRE recombinase pBOB-CAG-iCRE-s.d. (Addgene) or pA2UCOE-OGT vector, along with scramble or CREB siRNA as indicated.

    Immunohistochemistry:

    Article Title: High Fat-High Fructose Diet Elicits Hypogonadotropism Culminating in Autophagy-Mediated Defective Differentiation of Ovarian Follicles
    Article Snippet: Polyvinylidene difluoride (PVDF) membrane was purchased from Millipore, Bangalore, IN.Polyvinylidene difluoride (PVDF) membrane was purchased from Millipore, Bangalore, IN.. Primary antibodies ERα (SC-542), ERβ (SC-8974), AR (SC-815), GnRHR (SC-8682), PR (SC-398898), PRLR (SC-20992), wnt-2 (SC-514382), β-catenin (SC-797), Inhibin (SC-6306), CYP17A1(SC-46080), CYP11A1 (SC-18040), CYP19 (SC-14245), FSHR (SC-13935), LHR (SC-25828), StAR (SC-23523), 3βHSD (SC-30820), and 17βHSD (SC-26968) were purchased from Santacruz Biotechnology (TX, USA).. ATG5 (CST 12994S), ATG12 (CST 4180S), Beclin-1 (CST 3495S), LC3-II (CST 12741S), SQSMT/p62 (95697S), GSK-3β (CST 9315), and pGSK-3β (CST 9336), were purchased from Cell Signaling Technologies, MA, USA.ATG5 (CST 12994S), ATG12 (CST 4180S), Beclin-1 (CST 3495S), LC3-II (CST 12741S), SQSMT/p62 (95697S), GSK-3β (CST 9315), and pGSK-3β (CST 9336), were purchased from Cell Signaling Technologies, MA, USA.

    Article Title: The expressions of Wnt/β-catenin pathway-related components in brainstem gliomas.
    Article Snippet: Sections were then incubated with primary antibodies overnight at 4℃ in a humidified chamber.Sections were then incubated with primary antibodies overnight at 4℃ in a humidified chamber.. The primary antibodies against Wnt-1 (rabbit polyclonal antibody, Abcam, Cambridgeshire, UK), Wnt-2 (goat polyclonal antibody, R&D, Minnesota, USA) and C-myc (mouse monoclonal antibody, Santa Cruz, California, USA) were diluted to 1:50, 1:20 and 1:50, respectively.. The anti-β-catenin and antiKi-67 antibody (mouse monoclonal antibody, Millipore, Massachusetts, USA) were used at a 1:60 and 1:100 dilution.The anti-β-catenin and antiKi-67 antibody (mouse monoclonal antibody, Millipore, Massachusetts, USA) were used at a 1:60 and 1:100 dilution.

    Article Title: Dynamic O-GlcNAc modification regulates CREB-mediated gene expression and memory formation
    Article Snippet: Neurite outgrowth.Neurite outgrowth.. Neurons were electroporated with wild-type or S40A pLEMPRA, pMaxGFP, pcDNA3-Dkk-1-Flag or Ncad(intra) vectors and Wnt-2 (Santa Cruz), CRTC1 or scramble (Invitrogen) siRNA as indicated in the individual figures and then plated at a density of 25,000 neurons cm−2.. Neurons from B6.129-Ogttm1Gwh/J mice (Jackson Laboratories) were electroporated with pMaxGFP, the CRE recombinase pBOB-CAG-iCRE-s.d. (Addgene) or pA2UCOE-OGT vector, along with scramble or CREB siRNA as indicated.Neurons from B6.129-Ogttm1Gwh/J mice (Jackson Laboratories) were electroporated with pMaxGFP, the CRE recombinase pBOB-CAG-iCRE-s.d. (Addgene) or pA2UCOE-OGT vector, along with scramble or CREB siRNA as indicated.

    Expressing:

    Article Title: High Fat-High Fructose Diet Elicits Hypogonadotropism Culminating in Autophagy-Mediated Defective Differentiation of Ovarian Follicles
    Article Snippet: Polyvinylidene difluoride (PVDF) membrane was purchased from Millipore, Bangalore, IN.Polyvinylidene difluoride (PVDF) membrane was purchased from Millipore, Bangalore, IN.. Primary antibodies ERα (SC-542), ERβ (SC-8974), AR (SC-815), GnRHR (SC-8682), PR (SC-398898), PRLR (SC-20992), wnt-2 (SC-514382), β-catenin (SC-797), Inhibin (SC-6306), CYP17A1(SC-46080), CYP11A1 (SC-18040), CYP19 (SC-14245), FSHR (SC-13935), LHR (SC-25828), StAR (SC-23523), 3βHSD (SC-30820), and 17βHSD (SC-26968) were purchased from Santacruz Biotechnology (TX, USA).. ATG5 (CST 12994S), ATG12 (CST 4180S), Beclin-1 (CST 3495S), LC3-II (CST 12741S), SQSMT/p62 (95697S), GSK-3β (CST 9315), and pGSK-3β (CST 9336), were purchased from Cell Signaling Technologies, MA, USA.ATG5 (CST 12994S), ATG12 (CST 4180S), Beclin-1 (CST 3495S), LC3-II (CST 12741S), SQSMT/p62 (95697S), GSK-3β (CST 9315), and pGSK-3β (CST 9336), were purchased from Cell Signaling Technologies, MA, USA.

    Article Title: The expressions of Wnt/β-catenin pathway-related components in brainstem gliomas.
    Article Snippet: Sections were then incubated with primary antibodies overnight at 4℃ in a humidified chamber.Sections were then incubated with primary antibodies overnight at 4℃ in a humidified chamber.. The primary antibodies against Wnt-1 (rabbit polyclonal antibody, Abcam, Cambridgeshire, UK), Wnt-2 (goat polyclonal antibody, R&D, Minnesota, USA) and C-myc (mouse monoclonal antibody, Santa Cruz, California, USA) were diluted to 1:50, 1:20 and 1:50, respectively.. The anti-β-catenin and antiKi-67 antibody (mouse monoclonal antibody, Millipore, Massachusetts, USA) were used at a 1:60 and 1:100 dilution.The anti-β-catenin and antiKi-67 antibody (mouse monoclonal antibody, Millipore, Massachusetts, USA) were used at a 1:60 and 1:100 dilution.

    Article Title: Dynamic O-GlcNAc modification regulates CREB-mediated gene expression and memory formation
    Article Snippet: Neurite outgrowth.Neurite outgrowth.. Neurons were electroporated with wild-type or S40A pLEMPRA, pMaxGFP, pcDNA3-Dkk-1-Flag or Ncad(intra) vectors and Wnt-2 (Santa Cruz), CRTC1 or scramble (Invitrogen) siRNA as indicated in the individual figures and then plated at a density of 25,000 neurons cm−2.. Neurons from B6.129-Ogttm1Gwh/J mice (Jackson Laboratories) were electroporated with pMaxGFP, the CRE recombinase pBOB-CAG-iCRE-s.d. (Addgene) or pA2UCOE-OGT vector, along with scramble or CREB siRNA as indicated.Neurons from B6.129-Ogttm1Gwh/J mice (Jackson Laboratories) were electroporated with pMaxGFP, the CRE recombinase pBOB-CAG-iCRE-s.d. (Addgene) or pA2UCOE-OGT vector, along with scramble or CREB siRNA as indicated.



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    Image Search Results


    HA-induced activation of the canonical Wnt pathway upregulates FOSL2 to expand the dNK1-like subpopulation. (A) Analysis of transcriptional activity and expression levels of transcription factors in three dNK subsets using scRNA-seq data from normal decidual tissue ( n = 11). (B) Transcriptional activity and expression levels of FOSL2 in decidual tissues from normal pregnancy ( n = 5) and RSA groups ( n = 3). (C) Changes in NK92MI cells of the five transcription factors ( STAT3, MAFB, HES1, FOSL2, ETV5 ) characterized by high transcriptional activity and expression in the dNK1 subset following HMW-HA treatment, as determined by RT−qPCR ( n = 6 per group). (D) FOSL2 expression in NK92MI cells following HMW-HA treatment, assessed by WB ( n = 3 per group). (E) Wnt1 and β-catenin protein expression in NK92MI cells treated with or without HMW-HA in the presence or absence of CD44 blockade ( n = 3 per group). (F) FOSL2 expression in HA−treated NK92MI cells after addition of CD44−blocking antibody or the Wnt pathway inhibitor IWP−2 to the culture system ( n = 3 per group). Data are expressed as mean ± SD; * P < 0.05; ** P < 0.01; ns, not significant. EP, early pregnancy; SA, spontaneous abortion; RSA, recurrent spontaneous abortion.

    Journal: Frontiers in Immunology

    Article Title: Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

    doi: 10.3389/fimmu.2026.1777567

    Figure Lengend Snippet: HA-induced activation of the canonical Wnt pathway upregulates FOSL2 to expand the dNK1-like subpopulation. (A) Analysis of transcriptional activity and expression levels of transcription factors in three dNK subsets using scRNA-seq data from normal decidual tissue ( n = 11). (B) Transcriptional activity and expression levels of FOSL2 in decidual tissues from normal pregnancy ( n = 5) and RSA groups ( n = 3). (C) Changes in NK92MI cells of the five transcription factors ( STAT3, MAFB, HES1, FOSL2, ETV5 ) characterized by high transcriptional activity and expression in the dNK1 subset following HMW-HA treatment, as determined by RT−qPCR ( n = 6 per group). (D) FOSL2 expression in NK92MI cells following HMW-HA treatment, assessed by WB ( n = 3 per group). (E) Wnt1 and β-catenin protein expression in NK92MI cells treated with or without HMW-HA in the presence or absence of CD44 blockade ( n = 3 per group). (F) FOSL2 expression in HA−treated NK92MI cells after addition of CD44−blocking antibody or the Wnt pathway inhibitor IWP−2 to the culture system ( n = 3 per group). Data are expressed as mean ± SD; * P < 0.05; ** P < 0.01; ns, not significant. EP, early pregnancy; SA, spontaneous abortion; RSA, recurrent spontaneous abortion.

    Article Snippet: To determine the signaling pathways involved, we used an anti-CD44 blocking antibody (30 μg/mL; BioxCell, BE0262) to block CD44, and IWP-2 (50 μM; MedChemExpress, HY-13912) to inhibit the canonical Wnt pathway.

    Techniques: Activation Assay, Activity Assay, Expressing, Quantitative RT-PCR, Blocking Assay